Naslov
Advances in methods for the determination of biologically relevant lipid peroxidation products

Autori
Spickett, Corinne M ; Wiswedel, Ingrid ; Siems, Werner ; Žarković, Kamelija ; Žarković, Neven

Izvornik
Free radical research (1071-5762) 44 (2010), 10; 1172-1202

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
oxidative stress; oxysterols; F2 isoprostanes; 4-hydroxynonenal; aldehydes; mass spectroscopy; ELISA; immunohystochemistry

Sažetak
Lipid peroxidation is recognized to be an important contributor to many chronic diseases, especially those of an infl ammatory pathology. In addition to their value as markers of oxidative damage, lipid peroxidation products have also been shown to have a wide variety of biological and cell signalling effects. In view of this, accurate and sensitive methods for the measurement of lipid peroxidation products are essential. Although some assays have been described for many years, improvements in protocols are continually being reported and, with recent advances in instrumentation and technology, highly specialized and informative techniques are increasingly used. This article gives an overview of the most currently used methods and then addresses the recent advances in some specifi c approaches. The focus is on analysis of oxysterols, F 2 -isoprostanes and oxidized phospholipids by gas chromatography or liquid chromatography mass spectrometry techniques and immunoassays for the detection of 4-hydroxynonenal

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA