Naslov
An efficient method for concentration and purification of rubella virus using monolithic chromatographic supports

Autori
Forčić, Dubravko ; Brgles, Marija ; Ivančić, Jelena ; Šantak, Maja ; Halassy, Beata ; Barut, Miloš ; Jug, Renata ; Markušić, Maja ; Štrancar, Aleš

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts, MSS2010 - 4th Monolith Summer School & Symposium / Štrancar, Aleš - , 2010, 36-36

Skup
MSS2010 - 4th Monolith Summer School & Symposium, Applications in Biochromatography, Bioconversion and Solid Phase Synthesis

Mjesto i datum
Portorož, Slovenija, 29.05.2010. - 02.06.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
rubella ; purification ; monoliths ; chromatography

Sažetak
In order to produce quality as well as economically acceptable viral vaccine a development of simple and efficient method for purification and concentration of viral particles is required. Conventional procedures for virus concentration and purification are based on cycles of differential centrifugation, clarification with organic solvent and precipitation, and ultrafiltration. Such methods require a longer procedure time, expensive reagents and equipment and are not useful for large sample volumes. In recent years chromatography has become the method of choice for efficient virus concentration and purification. The aim of this study was to establish efficient method for concentration and purification of live rubella viruses by keeping high viability based on CIM monolithic chromatographic support. Presented results demonstrate successful binding, separation, purification and/or concentration of rubella viruses by CIM monoliths.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija

POVEZANOST RADA