Naslov
Intraspecies variability in Vipera ammodytes ammodytes venom related to its toxicity and immunogenic potential

Autori
Halassy, Beata ; Brgles, Marija ; Habjanec, Lidija ; Lang Balija, Maja ; Kurtović, Tihana ; Marchetti-Deschmann, Martina ; Križaj, Igor ; Allmaier, Guenter

Izvornik
Comparative biochemistry and physiology. C. Toxicology & pharmacology (1532-0456) 153 (2011), 2; 223-230

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Vipera ammodytes ammodytes venom; Ammodytoxin; Monolithic columns; Capillary electrophoresis-on-a-chip; ELISA; Lethal toxicity

Sažetak
Vipera ammodytes is the most venomous European snake, whose venom has been used as antigen for immunization of antivenom-producing animals. Same as venom of any other snake, it is a complex mixture of proteins, peptides and other compounds which biochemical and pharmacological variability has been demonstrated at inter- and intraspecies level. In this work we demonstrated intraspecific variability between 8 venom production batches using both the conventional and the new methodology. Moreover, in contrast to literature on different venoms’ variability, for the first time we were able to select those biochemical differences that are related to and give information on the venom’s toxicity and immunogenicity. We have shown that methods quantifying ammodytoxin (the most toxic compound identified so far in the Vipera ammodytes ammodytes venom) content of the venom clearly distinguish between high and low immunogenic venoms.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti, Biotehnologija

POVEZANOST RADA