Naslov
Novel Nonenzymatic Modifications of Lens Crystallins by Vitamin C Target Arginine Residues

Autori
Nemet, Ina ; Fan, Xingjun ; Monnier, Vincent M.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Skup
The Association for Research in Vision and Ophthalmology ARVO, annual meeting

Mjesto i datum
Fort Lauderdale (FL), Sjedinjene Američke Države, 02.05.2010. - 06.05.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Nonenzymatic Modifications; Lens Crystallins; Aggregation; Cataractogenesis; Vitamin C; Sugars; Arginine; Aging

Sažetak
Lens crystallins are prone to chemical modifications that affect chaperone function and favor toward aggregation and cataractogenesis. Vitamin C degradation products participate significantly in these chemical pathways in the lens. However, the products described so far can be formed from sugars other than Vitamin C and therefore do not reflect the specific role of Vitamin C in the modification. Here we characterized the structure of novel crystallin ascorbylation adducts and crosslinks with target amino acids and lens crystallins in vitro and from mice expressing the human vitamin C transporter 2 (hSCVT2) in the lens. Novel products of protein modifications by Vitamin C which target arginine in crystallins were characterized and identified in vitro and in vivo systems. They could destabilize arginine residues and contribute toward impaired chaperone function during aging.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA