Naslov
Morphological features and isoenzyme characterization of endosymbiotic algae from green hydra

Autori
Kovacevic, Goran ; Radic, Sandra ; Jelencic Biserka ; Posilovic, Hrvoje ; Pevalek-Kozlina Branka ; Kalafatic, Mirjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
31st Congress of the International Limnological Society

Mjesto i datum
Cape Town, Južnoafrička Republika, 15.08.2010. - 20.08.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
green hydra; endosymbiotic alga; isoenzymes; morphometry

Sažetak
Morphological features and isoenzyme characterization of permanently isolated endosymbionts from green hydra have been undescribed until now. Morphological characteristics (cell area, cell radius, chloroplast area) and isoenzyme patterns of esterase (EST), peroxidase (POX), and catalase (CAT) of aposymbiotic algae from green hydra (Cx) and from reference species Chlorella kessleri (Ck) and Chlorella vulgaris (Cv) were compared. Changeable morphology of isolated algae determined by light and electron microscopy was noticed. Results obtained by the native anodic electrophoresis suggest complex relations within the Chlorella genus. The greatest isoenzyme diversity was noted in EST patterns of isolated endosymbionts. Due to their much higher heterogeneity, EST isoenzymes seemed to be more suitable genetic markers for identification of different Chlorella species than CAT and POX isoenzymes. Morphological characteristics of researched species confirmed the interspecific diversity noticed by isoenzyme analysis.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA