Naslov
Comparison of mammalian serum esterases hydrolyzing acylated monosaccharides

Autori
Krstanović, Marina ; Tomašić, Jelka ; Tomić, Srđanka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
20th Anniversary Meeting of Croatian Biochemical Society / - , 1996

Skup
20th Anniversary Meeting of Croatian Biochemical Society

Mjesto i datum
Zagreb, Hrvatska, 18.10.1996. - 19.10.1996

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
mammalian serum ; esterases ; monosaccharides

Sažetak
Esterases of different specifity and activity are present in mammalian sera. In our previous studies we have described and characterized the esterases which could be used for deacylation of pivaloylated and acylated monosaccharides. The aim of the present study was to compare the activities of esterases originating from different mammals, as well as human serum. The deacylation was monitored throughout the experiments using methyl 2, 6-di-O- pivaloyl-a-D-(U-14C)glucopyranoside (2, 6-DP)as the representative substrate. Rabbit and guinea pig sera exhibited high esterase activities (characterized as carboxyl esterases). More detailed chromatgraphic analysis revealed the marked difference between these two sources ; rabbit serum contained one major peak which preferentially hydrolysed 6-O-pivaloyl group in 2, 6-DP, while in guinea pig serum two prominent peaks of activity were detected and one preferentially hydrolysed 2-O-pivaloyl group. Even more striking difference was noticed when human and sheep sera were examined, since the major event observed was the migration of 2-O-pivaloyl group to the position 4- in the sugar molecule, followed by very slow deesterification.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA