Naslov
Multiplication of HVT FC-126 (Herpesvirus Turkey) virus in the kidney cell lines of no avian origin

Autori
Filipič, Bratko ; Gottstein, Željko ; Sladoljev, Srećko ; Cencič, Avrelija ; Koren, Srečko ; Ciglar Grozdanić, Irena ; Mazija, Hrvoje

Izvornik
Acta Veterinaria (Beograd) (0567-8315) 60 (2010), 4; 381-389

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
adult pig kidney cell line ; HVT FC-126 ; Gerbil kidney cell line ; Marek’s disease

Sažetak
The presented experiments were aimed to cultivate and multiplicate HVT FC-126 in the PLA (Adult pig kidney) and GL-4 (Gerbil kidney) cell lines. Two different HVT FC-126 vaccine strains were used: Marikal SPF (Veterina d.o.o., Croatia) and Lyomarex (Merial, USA). They were adapted to the PLA and GL-4 cell lines. After adaptation, they were titrated on PLA (TCID50 2^4.23) and GL-4 (TCID50 2^4.96). On both cell lines they show similar CPE (cytophatic effect). The difference between them was detected using Real Time PCR, which was also positive by agarose gel analysis for the virus contained in Lyomarex, but not in the Marikal SPF. It can be concluded that both cell lines are sensitive to HVT FC-126 and the virus can be multiplied in high titers though much lower than in the calf intestinal epithelial cell line (CIEB) cells (TCID50 2^7.93).

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina

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