Naslov
Influence of Aspergillus flavus mycelium pre-treatment on ultrasonic disintergration efficiency

Autori
Šarkanj, Bojan ; Kovač, Tihomir ; Strelec, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of Microbes in Industry and Environment 2010, Programme and abstracts / Frece, Jadranka ; Kos, Blaženka ; Mrša, Vladimir - Zagreb : Hrvatsko mikrobiološko društvo, 2010, 129-129

ISBN
978-953-7778-00-2

Skup
Central European Symposium on Industrial Microbiology and Microbial Ecology, Power of Microbes in Industry and Environment 2010

Mjesto i datum
Malinska, Hrvatska, 22.09.2010. - 25.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Aspergillus flavus; ultrasonic disintegration; catalase activity; protein concentration

Sažetak
Ultrasonic disintegration is not among the most studied methodologies used for the purpose of cell disruption, according to the literature. The effect of Aspergillus flavus mycelium pre-treatment on ultrasonic disintegration efficiency, as well as optimisation of ultrasonic disintegration conditions for complete release of intracellular components without protein denaturation and loss of enzyme activity, was investigated. The results of ultrasonic disintegration monitored by microscopic analysis, protein quantification and determination of catalase activity were used to define a set of optimal working conditions: pre-treatment application of both quartz sand and lysozyme degradation of cell wall followed by ultrasonic processing for 5 minutes at full cycle and maximal amplitude. Under such conditions of ultrasonic homogenisation, extract obtained after 5 minute homogenization and extract of sediment additionally homogenized for 2 minutes contained a total of 87 % proteins, and 88 % enzyme activity.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

POVEZANOST RADA