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A q u a t i c s e d i m e n t s a r e t h e m a i n s i n k f o r a n t h r o p o g e n i c p o l l u t a nts which are known to induce versatile toxic effects in marine and freshwater organisms, among which the genotoxic ones are considered to be of particular significance. It is therefore important to investigate negative effects of such contaminated sediments on biota since this kind of information can serve as a warning of possible impacts on the entire aquatic environment. The main goal of this study was to test the usefulness of the Comet assay in the PLHC-1 hepatoma fish cell line as a tool for detecting the presence of genotoxic compounds in contaminated marine sediments. For all of the analysed sediment extracts a concentration dependent genotoxic effect was observed. The sediment with the highest observed genotoxic potential was additionally extracted using various solvents in order to test which class of compounds, according to their polarity, is mostly responsible for the observed genotoxic effect. Non-polar solvents (cyclohexane and dichloromethane) yielded stronger genotoxic effect but t ( * D F \ ^
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he highest level of DNA damage was determined after exposure to sediment extract obtained with the solvent mixture dichloromethane/methanol which extracts a wide range of contaminants. Our results indicate that the PLHC-1 cell line is a suitable in vitro model in sediment genotoxicity assessment and encourage the use of fish cell lines as versatile tools in ecogenotoxicology.
Keywords: Comet assay, PLHC-1 cell line, marine sediments, DNA damage, extraction solvent
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