Naslov
Spectrophotometric analysis of flavonoid-DNA interactions and DNA damaging/protecting and cytotoxic potential of flavonoids in human peripheral blood lymphocytes

Autori
Rusak, Gordana ; Piantanida, Ivo ; Mašić, Lozika ; Kapuralin, Katarina ; Durgo, Ksenija ; Kopjar, Nevenka

Izvornik
Chemico-biological interactions (0009-2797) 188 (2010), 1; 181-189

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
apigenin; kaempferol; luteolin; DNA; lymphocyte; cytoprotective/genoprotective effects

Sažetak
The ability of luteolin, kaempferol and apigenin to bind to calf thymus (ct)-DNA, mode of action and stability of flavonoids in buffer were investigated. Spectrophotometric analysis revealed a rapid degradation of apigenin in an aqueous medium, while kaempferol and luteolin were stable for 24 h upon dissolution in water. Spectrophotometric study of the interactions of kaempferol and luteolin with calf thymus DNA suggests classic intercalation as their dominant binding mode to DNA. Cytotoxicity/genotoxicity and cytoprotective/genoprotective effects of flavonoids in non-stressed and hydrogen peroxide stressed human peripheral lymphocytes were investigated using the fluorescent dye exclusion method and alkaline comet assay. Flavonoids revealed significant genoprotective effects in hydrogen peroxide stressed cells and in cells submitted to longer incubation in the cell culture medium. Luteolin, followed by apigenin and kaempferol, was shown to be the most effective in protecting DNA from oxidative damage induced by hydrogen peroxide. However, the investigated flavonoids also induced DNA damage, indicating their prooxidative capacity. The balance between the protection of DNA from oxidative damage and prooxidative effects was strongly dependent on flavonoid concentration and the incubation period.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

Napomena
Rad je kao poster prezentiran na skupu 31st Annual Meeting of the German Society for Cell Biology ; sažetak objavljen u European Journal of Cell Biology 87 (2008) (S1) ; http://www.sciencedirect.com/science/article/pii/S0171933508000149 ; DOI: doi:10.1016/j.ejcb.2008.01.004.

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