Naslov
Accumulation of clenbuterol residues in different pigmented mouse hair

Autori
Pleadin, Jelka ; Vulić, Ana ; Stojković, Ranko ; Perši, Nina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the XIIth International Congress of Toxicology ; u: Toxicology Letters 196 (2010) (S1) S1-S352 ; P306 Analytical Toxicology ; P306-010 / - , 2010, S295-S295

Skup
International Congress of Toxicology (12 ; 2010)

Mjesto i datum
Barcelona, Španjolska, 19.07.2010. - 23.07.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
clenbuterol; accumulation; residues; mouse; hair

Sažetak
Previous studies have indicated residual clenbuterol as a beta2-adrenergic agonist to persist in pigmented tissues such as hair for a longer period of time than in other matrices due to the matrix component or residual melanin. Also, there are reports on fair-colored hair to accumulate clenbuterol at a considerably lower level than dark hair. The aim of the present study was to investigate the effect of different hair pigmentation on clenbuterol accumulation, i.e. to compare the level of clenbuterol residues in white and black hair of mice upon repeat anabolic dose administration. The study included laboratory-bred white (n=60) and black (n=60) adult male (BALB/c) mice divided into four groups of 30 mice. Treated animals were administered clenbuterol per os (by a probe) in a dose of 2.5 mg/kg body weight once daily for 28 days, whereas control group were left untreated. Clenbuterol concentration in hair samples (white and black) collected on days 0, 15 and 30 was determined by use of previously validated methods of enzyme-linked immunosorbent assay (ELISA) as a screening method and liquid chromatography tandem mass spectrometry (LC-MS/MS) as a confirmatory method. In black hair, residual clenbuterol concentration was 2- to 3-fold that measured in white hair, decreasing at a comparable rate throughout the withdrawal period. Study results pointed to high accumulation of clenbuterol residues in both pigmented and non-pigmented mouse hair, suggesting dark hair to have a significantly higher accumulation potential for clenbuterol residues relative to fair hair, thus being more suitable as a matrix in the control of clenbuterol abuse as an anabolic.

Izvorni jezik
Engleski

Napomena
Doi:10.1016/j.toxlet.2010.03.932

POVEZANOST RADA