Naslov
CAN WE PREDICT PROGRESSION OF CERVICAL LESION BY P16INK IMMUNOASSAY?

Autori
Krivak Bolanca I., Sentija K., Katalenić Simon S.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Acta Cytologica / - , 2007

Skup
16th International congress of Cytology

Mjesto i datum
Vancouver, Kanada, 13.05.2007. - 17.05.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cervical dysplasia; clinical behavior; cytology; p16INK4a

Sažetak
One of the most important and mayor etiological factor for cervical carcinogenesis is persistant papilloma infection. It is well known fact that almost two thirds of LSIL (low grade squamous intraepithelial lesion) spontaneously regress, however it is not predictable which cases of LSIL will progress. Aim of the study is to predict the progression of cervical lesion by detecting p16INK in abnormal smear before progression started. Each of 21 patients included in the study underwent conisation and had histological verificationof high grade lesion (HSIL). All patients´archived smears were analysed retrospectively. Smear following at least two previous abnormal smears, was decolourised, immunoassayed and analysed. Results were classified as positive/negative, by morphology of cells and by intensity of staining reaction. Control group consisted of 10 patients with cytology and HPV-negative smears. Positive reaction in smears was found in 80, 95% (17/21) before progression started. In eleven patients follow up started with CIN I, six have had CIN II and one patient started with ASCUS (atypical squamous cell of undetermined significance). Among CIN I smears, p16 was positive 81, 8% (9/11), in group with CIN II positivism was 88, 88% (8/9) with more intense staining than in CIN I. In ASCUS smear result was negative. In CIN I and CIN II / p16 positive smears, stained cells from deeper layer was found in 81% (9/11) and 88% (8/9), respectively. In contlol group only one smear was slighty p16 positive. Conclusion: Positivism of p16INK in abnormal smears before the follow up started suggests that we can predict progressing of the disease. Finding p16 positive cells from deeper layer of epithelium suggest that these smears were under diagnosed. Therefore to avoid this mistake p16INK should be incorporated in routine laboratory practice for analysis and follow up of LSIL smears.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA