Naslov
Early changes in frequency of peripheral blood lymphocyte subpopulations in severe traumatic brain-injured patients

Autori
Mrakovčić-Šutić, Ines ; Sotošek Tokmadžić, Vlatka ; Laškarin, Gordana ; Mahmutefendić, Hana ; Lučin, Pero ; Župan, Željko ; Šustić, Alan

Izvornik
Scandinavian journal of immunology (0300-9475) 72 (2010), 1; 57-65

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
severe traumatic brain injury; T cells; NK cells; NKT cells; B cells

Sažetak
Infections are leading causes of increased morbidity and mortality of severe traumatic-brain injured (STBI) patients. The mechanism underlying the susceptibility to the infections is still unexplained. The purpose of the study was to investigate changes in frequency of leukocytes subpopulations in peripheral blood of patients with STBI during the course of intensive care treatment. Twenty patients with STBI were included in the study. Healthy age- and sex- volunteers served as control. Peripheral blood samples were taken from these patients at day 1, 4 and 7 and peripheral blood mononuclear cells (PBMC) were isolated. The percentage of T, B lymphocyte, NK and NKT cells as well as monocytes were analysed by simultaneous detection of surface antigens using fluorochrome conjugated monoclonal antibodies. The two major subsets of T lymphocytes (CD3+CD56-CD4+ and CD3+CD56-CD8+) and NK cells (CD3-CD56+dim and CD3-CD56+bright) were also analysed by flow cytometry. Extracranial infections were presented in 55 % STBI patients. At day 4, the percentage of T lymphocytes with cytotoxic phenotype significantly diminished and their numbers restored at day 7. The frequency of NKT cells showed the identical time dependent pattern, whereas the percentage of NK cells diminished on day 4 but did not restore after 7 days. The frequency of B lymphocytes did not change significantly during the time investigated, whereas the percentage of monocytes increased immediately after the injury and gradually diminished. The decrease of cells with cytotoxic phenotype might explain high incidence of susceptibility to infection of patients with STBI.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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