Naslov
Gibberellins and the coordination of flower development in the Christmas rose (Helleborus niger L.)

Autori
Ayele, Belay T. ; Magnus, Volker ; Mihaljević, Snježana ; Prebeg, Tatjana ; Čož-Rakovac, Rozelinda ; Ozga, Jocelyn A. ; Reinecke, Dennis M. ; Mander, Lewis N. ; Kamiya, Yuji ; Yamaguchi, Shinjiro ; Salopek-Sondi, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts for the 20th Annual Meeting of the International Plant Growth Substances Association / - Tarragona : International Plant Growth Substances Association, 2010, 138-138

Skup
20th Annual Meeting of the International Plant Growth Substances Association

Mjesto i datum
Tarragona, Španjolska, 28.06.2010. - 02.07.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Christmas rose (Helleborus niger L.); Flower and fruit development; Gibberellins; LC-MS/MS analyses; plant hormones

Sažetak
Gibberellins (GAs) were identified and quantified during flower and fruit development in the Christmas rose (Helleborus niger L.), which differs from other popular model species in two ways (1) following anthesis the initially white or rose sepals turns green, which persists until fruit ripening, (2) the seed is shed before embryo maturation. Using an LC-MS/MS system, we identified GA1 and GA4 as major bioactive GAs in fruit and sepal tissues. In the fruits, the endogenous amounts of GA4 peaked during rapid pericarp and embryo growth whereas that of GA1 during the subsequent period of rapid nutrient deposition in the seeds and continued pericarp enlargement. In the sepals, the flux through the GA biosynthetic pathway was highest prior to the light green stage when the photosynthetic system was induced. The greening of unfertilized, depistillated, and deseeded flowers was less than the seed-bearing controls ; and the greening (chlorophyll accumulation) could be restored by treatment of the deseeded fruit with exogenous GA1 and GA4. The sepals of unfertilized and depistillated flowers indeed contained very low levels of GA4 and gradually decreasing levels of GA1. However, the concentrations of their precursors and metabolites were less affected. These data suggest that a signal(s) from the fruit stimulates GA biosynthesis and in turn greening in the sepals.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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