Naslov
Developmental aspects of long-term callus culture of Vicia faba L.

Autori
Jelaska, Sibila ; Pevalek, Branka ; Papeš, Dražena ; Devide, Zvonimir

Izvornik
Protoplasma (0033-183X) 105 (1981); 285-292

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
growth medium optimization ; long-term callus cultures ; ploidy level estimation ; rhizogenesis ; Vicia faba

Sažetak
Vicia faba callus line (VFS1), isolated from explants of immature embryo, grew satisfactoriliy on Murashige and Skoog complete medium with 1.38 µM 2, 4-D, or with 0.92 µM 2, 4-D to which 1.0 µM kinetin was added. It also grew well on the B5 modified medium containing 2.3 µM 2, 4-D and 25.0 µM kinetin. On the last of these media the cultures grew more uniformly and without necrosis. They also showed diminishing variation in poliploidy in favour of diploids and corresponding aneuploids (hypodiploids). After being cultured for nearly three years on MS containing 1.38 µM 2, 4-D, 8-33% of cultures of VFS1 were able to regenerate roots when transferred to either MS half strength with 5.37 µM NAA, or to a medium without 2, 4-D, or else to media with addition of kinetin only (in various concentrations).

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA