Naslov
Infekcije s Mycobacterium caprae i Mycobacterium bovis u goveda u Hrvatskoj
(Mycobacterium capraae and Mycobacterium bovis infection in cattle in Croatia)

Autori
Špičić, Silvio ; Zdelar-Tuk, Maja ; Duvnjak, Sanja ; Račić, Ivana ; Kompes, Gordan ; Habrun, Boris ; Cvetnić, Željko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book, ESM, Annual Congress of the European Society of Mycobacteriology / Darja Kušar, Urška Bidovec-Stojkovič - Ljubljana, 2010, PP-81

ISBN
978-961-6633-28-4

Skup
Annual Congress of the European Society of Mycobacteriology

Mjesto i datum
Slovenija, 04.07.2010. - 07.07.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Mycobacterium caprae; goveda; Mycobacterium bovis; Hrvatska
(Mycobacterium caprae; cattle; Mycobacterium bovis; Croatia)

Sažetak
Introduction Bovine tuberculosis (BTB) is caused by Mycobacterium bovis and closely related mycobacteria of the Mycobacterium tuberculosis complex. BTB has a major economic impact on livestock productivity, can persist in wildlife and thus affect entire ecosystems, and it is of public health concern due to its zoonotic potential. In the period from 2001 to 2006 we monitored the circulation of tuberculosis in cows within the regular annual tuberculosis control in Republic of Croatia. Materials and methods Cattle older then 6 weeks were subjected to tuberculin skin test with bovine tuberculin. After 6 weeks suspecious and positive ones were retested by avian and bovine tuberculin (comparative method). Positive reactors were slaughtered and material for bacterial testing collected. Isolates were identified and typed by molecular methods: PCR detecting a gene for 65kD antigen, specific hybridization with GenoType CM and MTBC kits – Hain, Lifescience and MIRU-VNTR. Results During tested period positive reactions on bovine tuberculine were found in 95 (9.4%) and suspicious ones in 34 (3.4%) tested cows. Pathoanatomic examination upon slaughtering was carried out on 123 cows, which were found positive or suspicious with tuberculine testing. Mycobacteria were extracted from 83 (67.5%) cows in 6 counties. Mycobacterium (M.) caprae was isolated in 71 cows from 7 flocks, M. bovis was isolated from 11 cows from 4 flocks and 1 isolate belonging to M. avium complex. Conclusions According to mutual comparison of the genotypes and other epizootiological facts we determined the sources and routes of spreading the infection with types of M. bovis and M. caprae in cows. We also found M. caprae as a dominant couse of bovine tuberculosis in Croatia. The defined genotypes of our mycobacteria isolates are comparable to other results of this type in the world thus providing us the basis for further research regarding epizootiology and epidemiology.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA