Pregled bibliografske jedinice broj: 47435
Isolation and identification of quercetagetin 3'-methyl ether 7-O-beta-D-glukopyranoside, a flavonoid with antiphytoviral activity, from Centaurea rupestris L.
Isolation and identification of quercetagetin 3'-methyl ether 7-O-beta-D-glukopyranoside, a flavonoid with antiphytoviral activity, from Centaurea rupestris L. // Book of abstracts of the Silver Jubilee Meeting of the Croatian Biochemical Society (HB2000) / Floegel, Mirna (ur.).
Zagreb: Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2000. str. 123-123 (poster, domaća recenzija, sažetak, znanstveni)
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Naslov
Isolation and identification of quercetagetin 3'-methyl ether 7-O-beta-D-glukopyranoside, a flavonoid with antiphytoviral activity, from Centaurea rupestris L.
Autori
Rusak, Gordana ; Robinson, Neil
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of abstracts of the Silver Jubilee Meeting of the Croatian Biochemical Society (HB2000)
/ Floegel, Mirna - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2000, 123-123
Skup
Silver Jubilee Meeting of the Croatian Biochemical Society (HB2000)
Mjesto i datum
Zagreb, Hrvatska, 13.10.2000. - 15.10.2000
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
Centaurea rupestris; antiphytoviral activity; quercetagetin
Sažetak
The flavonoid which revealed a strong antiphytoviral activity against tomato bushy stunt tombusvirus was isolated from methanolic extract of the inflorescence of Centaurea rupestris L. (Asteraceae) and its chemical structure was elucidated. The isolation was started by means of column (silica gel 60) and preparative thin layer (aluminium sheets, silica gel 60, F254) chromatography. Final purification was achived by preparative HPLC. Isolated flavonoid was submitted to LC-MS analyses which demonstrated that isolated flavonoid had a molecular weight of 494. The flavonoid was assigned molecular formula C22H22O13 and it was submitted to a range of homonuclear and heteronuclear 1H and 13C NMR experiments. The 1HNMR spectrum exhibited for ring B 3H ABX system at Ô 7.00 (d, J = 8 Hz, H5'), 7.75 (dd, J = 8 and 2 Hz, H6' and 7.85
(d, J = 2 Hz, H2'). H8 occurred at Ô 7.00, consistent with a 7-0-glucoside and differentiating the compound from 6- or 4' - O – glycoside. The anomeric proton appeared at Ô 5.18, again suggesting 7-O- glycosilation and ruling out
3-glycosilation. Based on these spectral interpretation and comparasion with literature data, isolated flavonoid was identificated as quercetagetin 3' – methyl ether 7-O-Ň-D glucopyranoside.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Projekti:
119117
Ustanove:
Prirodoslovno-matematički fakultet, Zagreb
Profili:
Gordana Rusak
(autor)
