Naslov
Predominant type-2 response in infants with respiratory syncytial virus (RSV) infection demonstrated by cytokine flow cytometry

Autori
Bendelja, Krešo ; Gagro, Alenka ; Baće, Ana ; Lokar-Kolbas, Renata ; Krstulović-Hrešić, Vilka ; Draženović, Vlado ; Mlinarić-Galinović, Gordana ; Rabatić, Sabina

Izvornik
Clinical and experimental immunology (0009-9104) 121 (2000), 2; 332-338

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
respiratory syncytial virus; intracellular cytokines; flow cytometry; IL-4; interferon-gamma; lymphocytes

Sažetak
Acute RSV infection in infancy may produce some asthma-like symptoms and may be followed by a recurrent wheeze later in life. It has been proposed that RSV infection stimulates type-2 cytokine responses, resembles those found in atopy and asthma. Peripheral blood cells were obtained from RSV?infected infants (n=30) and healthy controls (n=10). After in vitro restimulation of the cells, intracellular IL-4 and interferon-gamma (IFN-g) were measured by flow cytometry. The cells from RSV?infected infants produced more IL-4 and less IFN-g than those from healthy controls. IL-4 production was more frequent in CD8 than in CD4 cells, and the bias towards IL-4 production was greatest in infants with mild infections, whereas IFN-g production increased with disease severity. Our conclusions are that RSV infection is associated with IL-4 production in peripheral T cells, and that peripheral blood in infants with severe disease may be depleted of cytokine-producing cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA