Naslov
Ammodytoxin content screening in the Vipera ammodytes ammodytes venom samples

Autori
Brgles, Marija ; Habjanec, Lidija ; Kurtović, Tihana ; Križaj, Igor ; Barut, Miloš ; Štrancar, Aleš ; Halassy, Beata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Book of Abstracts, MSS2010-4rd Monolith Summer School and Symposium / Štrancar, Aleš - , 2010, 43-43

Skup
MSS2010-4rd Monolith Summer School and Symposium, Applications in Biochromatography, Bioconversion and Solid Phase Synthesis

Mjesto i datum
Portorož, Slovenija, 30.05.2010. - 02.06.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Ammodytoxins; Capillary gel electrophoresis-on-a-chip; ELISA

Sažetak
Snake venoms are complex mixtures of more then 100 proteins, peptides and other compounds which biochemical and pharmacological variability has been demonstrated. These mixtures have been used as antigens for immunization of antivenom-producing animals (horses or sheep). Ammodytoxins (Atx) are potent neurotoxins, and the most toxic compounds isolated so far from the Vipera ammodytes ammodytes (Vaa) venom. The content of Atx in the Vaa venom is a good measure of its immunogenicity and toxicity. The development of the cation exchange chromatographic method using CM-CIM for extraction and fast separation of highly basic venom components (including Atxs) will be presented. The usefulness of the method for the screening of venom samples, in comparison to several other methods, including capillary gel electrophoresis-on-a-chip and newly established ELISA, will be reported and discussed.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Biotehnologija

POVEZANOST RADA