Naslov
Selection of probiotic strains from Croatian traditional fresh cheese

Autori
Leboš Pavunc, Andreja ; Kos, Blaženka ; Beganović, Jasna ; Gjuračić, Krešimir ; Šušković, Jagoda

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
5th Central European Congress on Food

Mjesto i datum
Bratislava, Slovačka, 19.05.2010. - 22.05.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antimicrobial activity; traditional fresh cheese; autochthonous lactic acid bacteria; probiotics; simulated gastrointestinal tract conditions
(antimicrobial activity; traditional fresh cheese; autochthonous lactic acid bacteria; probiotics; simulated gastrointestinal conditions)

Sažetak
The aim of this study was to screen lactic acid bacteria isolated from Croatian traditional fresh cheese, with potential probiotic properties. Fifteen gram-positive, catalasa-negative and asporogenous bacterial strains were isolated and analysed. Eleven lactic acid bacterial strains were characterized as mesophilic cocci and four as thermofilic lactobacilli. SDS-PAGE of whole cell proteins as well as PFGE and RAPD analysis have revealed that those are 2 different lactic acid bacteria strains, identified by API test and DNA fingerprinting using AFLP, as Lactobacillus fermentum A8 and Enterococcus faecium A7. These strains were resistant to low pH (pH = 2.0) and pepsin in simulated gastric juice, and to pancreatic enzymes and high bile salt concentration (3.0 mg/ml) in simulated small intestine juice. Both examined strains were susceptible to tested antibiotics with exception of vancomycine resistance of Lactobacillus fermentum A8 which is intrinsic resistance of some Lactobacillus strains. Strong autoaggregation and coaggregation abilities of two strains with different probiotic strains (Lactobacillus helveticus M92, Lactobacillus plantarum L4 and Enterococcus faecium L3) were obtained, while Lactobacillus fermentum A8 have also shown ability to coaggregate with testmicroorganisms (Staphylococcus aureus 3048, Escherichia coli 3014, Salmonella enterica serovar Typhimurium and Bacillus subtilis ATCC 6633), what could be important trait in the “competitve exclusion” of pathogens in gastrointestinal tract conditions. Additionally, Lactobacillus fermentum A8 strain have shown potential bacteriocinogenic activity against representatives of the similar bacterial species. Both strains were prepared as lyophilized cultures with 109 CFU/g and the viable cell count remained high during 12 months of storage.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA