Naslov
Genetic polymorphisms of 17 Y-Chromosomal short tandem repeat loci in Central Croatian population

Autori
Gršković, Branka ; Mršić, Gordan ; Polašek, Ozren ; Vrdoljak, Andro ; Merkaš, Siniša ; Anđelinović, Šimun

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Haploid DNA Markers in Forensic Genetics / Walther Parson and Lutz Roewer - Berlin, 2010

Skup
7th International Y Chromosome User Workshop

Mjesto i datum
Berlin, Njemačka, 22.04.2010. - 24.04.2010

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Y-chromosome; Central Croatian; 17 Y-Chromosomal Short Tandem

Sažetak
In forensics, Y-chromosome short tandem repeat (STR) haplotyping is used in human identification, paternity testing and sexual assault cases where Y-STRs provide a malespecific DNA profile. The reference Croatian Y-STR database is important for forensic purposes, especially because there is a lack of information about the genetic structure of Y-chromosome in some Croatian regions. The aim of this study was to describe the genetic structure of Y-chromosome in Central Croatian population. We carried out a statistical analysis of the data from previously performed genetic analysis collected during the routine forensic work by the Forensic Science Centre “Ivan Vučetić”. A total of 220 unrelated healthy men from Central Croatia were selected for the purpose of this study. Genomic DNA was extracted using Chelex procedure from FTA cards. Y-Chromosomal STRs were determined using the AmpFlSTR Yfiler PCR amplification kit. The haplotype frequencies were determined by direct counting and analyzed using Arlequin 3.1 and AMOVA analysis calculated with YHRD online analysis tool. A total of 212 haplotypes were identified, 204 of which were unique. Total haplotype diversity was 0.993. Locus diversity varied from 0.325 for DYS392 to 0.786 for DYS385. Discrimination capacity was 92.7%. Allele frequencies diversity was 0.6149. Intermediate alleles 17.2, 18.2 and 19.2 were found at DYS458 locus. The analysis of Y-chromosome variability among publicly available population samples from the YHRD.org website was based on a minimal European haplotype set. AMOVA analysis indicated that Central Croatian population was the closest to population sample from Croatian capital Zagreb and some population samples from Bosnia and Herzegovina with no paternal contribution from the other neighboring countries such as Slovenia and Austria. Further studies are needed in order to fulfill Croatian Y-STR database and to use this data in routine forensic work and to get information about genetic similarity between the neighboring populations.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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