Naslov
Magnesium concentration in mononuclear cells of COPD patients in stable phase

Autori
Ruljančić, Nedjeljka ; Popović-Grle, Sanja ; Rumenjak, Vlatko ; Sokolić, Božica ; Malić, Ana ; Mihanović, Mate ; Čepelak, Ivana.

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Dietary Magnesium:New Research

Urednik/ci
Yardley, Andrew W

Izdavač
Nova Science Publishers

Grad
New York (NY)

Godina
2008

Raspon stranica
1-9

ISBN
978-1-60692-109-8

Ključne riječi
ukupni magnezij, ionizirani magnezij, mononuklearne stanice, KOPB
(total magnesium, ionized magnesium, mononuclear cells, COPD)

Sažetak
Magnesium (Mg) is the most abundant intracellular divalent cation and free cytoplasmic Mg can modulate the activity of a number of cellular enzymes, including key enzymes in phospholipase C signalling pathway which are initiated by antigen receptors on lymphocytes. The concentration of Mg may change after the cell activation. Lymphocytes have a role in the pathogenesis of COPD and recent studies indicate their abnormal function. As result of the above findings we determined the concentrations of total magnesium (tMg) and ionised magnesium (iMg) in isolated mononuclear cells (MNC) by using group of 46 COPD stable phase patients (past smokers, current smokers and non-smokers) and 61 control subjects (24 healthy smokers and 37 healthy non-smokers). Due to the antagonism of magnesium towards calcium we determined in the same sample the concentrations of the total (tCa) and ionised calcium (iCa). We found an increased of biologically active iMg in isolated MNC of the COPD patients compared to the group oh healthy non-smokers (median 22.09 μmol/109 cells, CI 5.67-50.00 vs. median 17.02 μmol/109 cells, CI 10.84-38.89, p<0.05) and increased concentration of tCa (36.01 μmol/109 cells, CI 4.81-82.14 vs. 18.18 μmol/109 cells, CI 3.84-74.66, 17.99 μmol/109 cells, CI 2.77-33.33, p<0.05) compared to the group of healthy non-smokers and healthy smokers respectively. In isolated MNC of the patients the ratio of total calcium/total magnesium (tCa/tMg) was significantly increased (2.55, CI 0.57-6.33) compared to the group of healthy non-smokers (1.48, CI 0.20-6.22) and healthy smokers (1.51, CI 0.17-3.84). The MNC iMg value in COPD patients shows significant correlation with iCa (r=0.94, p=0.0000) and tMg with tCa (r=0.41, p=0.0237). Above study findings indicates that differential in the magnesium mobilization response has an impact on the resulting cellular response and will regulate cellular response to given stimulus.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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