Naslov
Developmental competence of bovine immature oocytes and quality of embryos derived from slaughterhouse ovaries or live donors by ovum pick up

Autori
Karadjole, Martina ; Getz, Iva ; Samardžija, Marko ; Maćešić, Nino ; Matković, Mario ; Makek, Zdenko ; Karadjole, Tugomir, Bačić, Goran, Dobranić, Tomislav, Poletto, Marko

Izvornik
Veterinarski arhiv (0372-5480) 80 (2010), 4; 445-454

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
heifer; oocyte; developmental competence

Sažetak
The aim of this research was to compare the developmental competence of oocytes recovered from slaughterhouse ovaries (SO) and those recovered in vivo by ovum pick up (OPU). OPU was performed in 6 donor heifers synchronized with PGF2 and stimulated with pFSH, twice a day during two days. OPU was repeated once a week for six consecutive weeks. The control group consisted of slaughterhouse-derived oocytes. The number of aspirated follicles, retrieved oocytes and oocyte recovery rate were recorded. Oocytes of grade 1 and 2 were matured, fertilized and cultured in vitro in SOFaaBSA until Day 9. The cleavage rates on Day 2, the total number of morulas and blastocysts on Day 7 and the numbers of hatched blastocysts on Day 9 were recorded. Differential staining of the inner cell mass (ICM) and trophectoderm cells (TE) were performed on randome sample of Day 7 blastocysts (n=24). Significantly more follicles were aspirated from SO (19.3 versus 11.5, P<0.05). This was reflected in significantly higher number of oocytes collected from the same group (12.6 versus 6.5, P<0.05). Higher proportion of OPU-derived oocytes reached the morula/blastocyst stage at Day 7 (44.7% vs. 29.9%, P<0.05) and hatched blastocyst stage at Day 9 (35.6% vs. 16.8%, P<0.05). OPU-derived embryos displayed significantly higher number (P<0.05) of ICM cells which was also reflected to higher proportion of ICM cells. The results demonstrate that oocytes recovered in vivo after OPU are more competent to develop to the blastocyst stage than those derived from slaughterhouse ovaries.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA