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Pregled bibliografske jedinice broj: 465097

PCR-quality DNA isolation from human bronchial aspirates and buccal and eyelid swabs by a simple procedure based on alkaline lysis

Vrabec Branica, Božica; Mitrečić, Dinko; Grgić, Sandra; Smojver Ježek, Silvana; Chalfe, N; Gajović, Srećko
PCR-quality DNA isolation from human bronchial aspirates and buccal and eyelid swabs by a simple procedure based on alkaline lysis // Genetic testing, 13 (2009), 6; 799-802 doi:10.1089/gtmb.2009.0066 (međunarodna recenzija, članak, znanstveni)

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Naslov
PCR-quality DNA isolation from human bronchial aspirates and buccal and eyelid swabs by a simple procedure based on alkaline lysis

Autori
Vrabec Branica, Božica ; Mitrečić, Dinko ; Grgić, Sandra ; Smojver Ježek, Silvana ; Chalfe, N ; Gajović, Srećko

Izvornik
Genetic testing (1090-6576) 13 (2009), 6; 799-802

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA ; isolation ; PCR ; lungs

Sažetak
There are only a few systematic reports about DNA extraction from routine diagnostic cytological specimens. An inevitable drawback of such techniques is increased spending of time and funds required for obligatory DNA purification. To implement a simple protocol for human DNA isolation from cytological specimens related to lung cancer, bronchial aspirates together with samples collected by swabbing of the inner cheek and eyelid were used. By combining alkaline and temperature lyses it was possible to isolate DNA solution ready for PCR in less than an hour. Testing the method used for amplification of sex chromatin gene fragments showed that it is highly efficient. The presented protocol preserves high-quality DNA that is suitable for PCR-based assays.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA

Projekti:
108-1081870-1902 - Uloga gena u diferencijaciji i plastičnosti središnjeg živčanog sustava miša (Gajović, Srećko, MZOS ) ( CroRIS)

Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Božica Vrabec-Branica (autor)

Avatar Url Srećko Gajović (autor)

Avatar Url Silvana Smojver-Ježek (autor)

Avatar Url Dinko Mitrečić (autor)

Poveznice na cjeloviti tekst rada:

doi go.galegroup.com online.liebertpub.com

Citiraj ovu publikaciju:

Vrabec Branica, Božica; Mitrečić, Dinko; Grgić, Sandra; Smojver Ježek, Silvana; Chalfe, N; Gajović, Srećko
PCR-quality DNA isolation from human bronchial aspirates and buccal and eyelid swabs by a simple procedure based on alkaline lysis // Genetic testing, 13 (2009), 6; 799-802 doi:10.1089/gtmb.2009.0066 (međunarodna recenzija, članak, znanstveni)
Vrabec Branica, B., Mitrečić, D., Grgić, S., Smojver Ježek, S., Chalfe, N. & Gajović, S. (2009) PCR-quality DNA isolation from human bronchial aspirates and buccal and eyelid swabs by a simple procedure based on alkaline lysis. Genetic testing, 13 (6), 799-802 doi:10.1089/gtmb.2009.0066.
@article{article, author = {Vrabec Branica, Bo\v{z}ica and Mitre\v{c}i\'{c}, Dinko and Grgi\'{c}, Sandra and Smojver Je\v{z}ek, Silvana and Chalfe, N and Gajovi\'{c}, Sre\'{c}ko}, year = {2009}, pages = {799-802}, DOI = {10.1089/gtmb.2009.0066}, keywords = {DNA, isolation, PCR, lungs}, journal = {Genetic testing}, doi = {10.1089/gtmb.2009.0066}, volume = {13}, number = {6}, issn = {1090-6576}, title = {PCR-quality DNA isolation from human bronchial aspirates and buccal and eyelid swabs by a simple procedure based on alkaline lysis}, keyword = {DNA, isolation, PCR, lungs} }
@article{article, author = {Vrabec Branica, Bo\v{z}ica and Mitre\v{c}i\'{c}, Dinko and Grgi\'{c}, Sandra and Smojver Je\v{z}ek, Silvana and Chalfe, N and Gajovi\'{c}, Sre\'{c}ko}, year = {2009}, pages = {799-802}, DOI = {10.1089/gtmb.2009.0066}, keywords = {DNA, isolation, PCR, lungs}, journal = {Genetic testing}, doi = {10.1089/gtmb.2009.0066}, volume = {13}, number = {6}, issn = {1090-6576}, title = {PCR-quality DNA isolation from human bronchial aspirates and buccal and eyelid swabs by a simple procedure based on alkaline lysis}, keyword = {DNA, isolation, PCR, lungs} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI

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