Naslov
Lower TLR8 expression restores in the convalescent phase upon acute RSV infection in infants

Autori
Bendelja, Krešo ; Vojvoda, Valerija ; Baće, Ana ; Turkalj, Mirjana ; Gagro, Alenka ; Mlinarić-Galinović, Gordana ; Rabatić, Sabina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts / Rabatić, Sabina - Zagreb, 2009, 16-16

Skup
2009 Annual meeting of Croatian Immunological Society

Mjesto i datum
Starigrad, Hrvatska, 01.10.2009. - 04.10.2009

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
RSV; toll like receptor; TLR3; TLR7; TLR8

Sažetak
Infants are particularly sensitive to different viral infections with emphasized detrimental effect of respiratory syncytial virus (RSV) inducing lower respiratory tract infections that could be related to inadequate antiviral immunity. Toll-like receptors (TLRs) are part of the innate immunity able to activate immune defense upon pathogen challenge. TLR3 and TLR8 recognize viral RNA and initiate cascade of antiviral mechanisms and develop specific immune reaction. Inadequate TLR3 and TLR8 engagement in innate immunity responses could result in inability to control RSV replication, to induce anti-viral cytokines and development of specific anti-RSV immune response. In our study specific monoclonal antibodies against human TLR3 and TLR8 were used to determine expression in monocytes from healthy infants, RSV-infected infants during acute infection and convalescent phase. Functional status of TLR's in monocytes was assessed by intracellular TNF-alpha and IL-10 staining upon TLR-specific stimulation. RSV-infected infants had lower percentages of TLR3 and TLR8 positive monocytes than healthy controls and RSV-infected infants in convalescent phase. Lower TLR8 expression during acute RSV-infection was accompanied by the lower TNF-alpha synthesis that substantially increased in the convalescent phase, whereas IL-10 synthesis was higher in acute phase and decreased post-infection. In conclusion, lower expression of TLR8 in acute RSV-infected infants could indicate insufficient participation of innate immune mechanisms that are responsible for the control of RSV replication and generation an effective immune response. Moreover, lower TLR8 expression might represent a specific RSV-mediated mechanism circumventing effective antiviral immune response.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA