Naslov
Effects of High Sucrose Diet, Gemfibrozil, or Their Combination on Plasma Paraoxonase 1 Activity and Lipids Level in Rats

Autori
Macan, Marija ; Vrkić, Nada ; Lucić Vrdoljak, Ana ; Radić, Božica ; Bradamante, Vlasta

Izvornik
Acta biochimica Polonica (0001-527X) 57 (2010), 3; 321-326

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
high sucrose diet; gemfibrozil; paraoxonase; lipids

Sažetak
We investigated the influence of high sucrose diet (HSD) after 3 and 5 weeks of administration on PON1 activity in plasma of normolipidemic rats and to determine the relationship between serum PON1 activity, triacylglycerides (TG), HDL and total cholesterol vs. the control group (CD). Because the data about the influence of gemfibrozil (GEM) on PON1 activity are controversial, we also investigated its effects (the administration in the 4th and 5th week in rats on HSD and CD) on plasma PON1 activity and lipids level in normolipidemic rats, and in rats with hypertriglyceridemia caused by HSD. Our results obtained in rats on HSD show the significant increase of plasma TGs levels by 47% (p<0.05) after 5 weeks of treatment, and PON1 activity by 32% and 21% (p<0.05) after 3 and 5 weeks, but without change in lipid levels vs. rats on CD. In the rats on CD and HSD, the addition of GEM caused the significant decrease of PON1 activity by 44% and 33%, while the significant decrease of TG level by 38% (p<0.05) was measured only in rats on CD. The effects of GEM on total, HDL and LDL- cholesterol in both groups of rats were typical for its action on lipoprotein metabolism. Because GEM in the rat’s liver stimulates proliferation of peroxisome, β oxidation, and production of H2O2, it is possible that the oxidative stress induced by GEM, causes damage of both hepatocytes and synthesis of PON1. Due to this reason, plasma PON1 activity was decreased after GEM treatment.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti, Farmacija

POVEZANOST RADA