Naslov
Loss of heterozygosity of selected tumor suppressor genes in human testicular germ cell tumors

Autori
Vladušić, Tomislav ; Hrašćan, Reno ; Vrhovac, Ivana ; Krušlin, Božo ; Gamulin, Marija ; Pećina-Šlaus, Nives ; Franekić-Čolić, Jasna

Izvornik
Pathology, research and practice (0344-0338) 206 (2010), 3; 163-167

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
loss of heterozygosity; germ cell tumors; tumor suppressor genes

Sažetak
Human testicular germ cell tumors (TGCTs) are histologically heterogenous neoplasms with a variable malignant potential. Two main groups of germ cell tumors occur in men: seminomas and nonseminomas. In the present study, a set of four tumor suppressor genes was investigated in testicular cancers. CDH1, APC, p53, and nm23-H1 genes were tested for loss of heterozygosity (LOH). Thirty-eight testicular germ cell tumors (17 seminomas and 21 nonseminomas) were analyzed by PCR using restriction fragment length polymorphism or the dinucleotide/tetranucleotide repeat polymorphism method. An allelic loss of p53 at exon 4 was detected in five nonseminomas, whereas LOH of p53 at intron 6 occurred in one of the seminoma and two of the nonseminoma samples. Allelic losses of the APC gene were present in three seminomas and one nonseminoma, whereas one seminoma and three nonseminomas showed LOH of CDH1. The analysis of allelic losses showed no common structural genetic alterations in tumor tissues, although a different pattern of LOH was observed between the two main histological groups of TGCTs. Copyright 2009 Elsevier GmbH. All rights reserved.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Biotehnologija

POVEZANOST RADA