Naslov
Depletion of CCR4+ and CXCR3+ T lymphocytes from peripheral blood in acute RSV infection

Autori
Vojvoda, Valerija ; Bendelja, Krešo ; Baće, Ana ; Čepin-Bogović, Jasna ; Gagro, Alenka ; Mlinarić- Galinović, Gordana ; Rabatić, Sabina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2009 Annual Meetting of the Croatian Immunological Society, Book of Abstracts / - , 2009, 21-21

Skup
2009 Annual Meetting of the Croatian Immunological Society

Mjesto i datum
Starigrad, Hrvatska, 01.10.2009. - 04.10.2009

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
RSV; chemokine; infants; IP-10; MIG; fractalkine; TARC; MDC

Sažetak
Undeveloped immune system in infants is considered to be responsible for the severe RSV infections in infants (i.e. bronchiolitis) since virus can skew antiviral type-1 to less effective type-2 immune reaction. Accumulation of lymphocytes in the lungs, during RSV infection, is mediated by type-1 chemokines (IP-10, MIG and fractalkine) through binding of CXCR3 and CX3CR1. Opposing type-2 chemokines (TARC and MDC) are responsible for migration of CCR4-positive lymphocytes involved in allergic immune responses. We postulate that RSV- infected infants produce elevated type-2 chemokines and compromise antiviral response. PBMC from RSV-, adenovirus/influenza-infected infants and RSV-infants collected 4-6 weeks after first sampling were simultaneously stained with antibodies for chemokine receptors (CCR4, CXCR3, CX3CR1) and lymphocyte subpopulation markers. Serum chemokines (TARC, MDC, IP-10, MIG, fractalkline) were determined by ELISA. CD4+ T- lymphocytes bearing CCR4 and CXCR3 were depleted from peripheral blood in RSV-infected infants, compared to infants with adenovirus/influenza infections. Interestingly, more B-lymphocytes of RSV-infected infants express CCR4, CXCR3 and CX3CR1 compared to adenovirus/influenza-infected infants. In convalescent phase, percentages of CCR4+ and CXCR3+ CD4+ T-lymphocytes increased while percentages of CCR4+, CXCR3+ and CX3CR1+ B- cells decreased. In acute phase of RSV infections, the predominant chemokines were TARC, MDC and IP- 10. In convalescent phase serum levels of TARC, MDC and IP-10 decreased. In conclusion, we observed a mixed type of chemokine production and chemokine receptors level during acute phase of RSV infection. Higher TARC levels and CCR4 positive lymphocytes could contribute to type-2 cytokine synthesis and confront effective type-1 cytokine-driven antiviral immune response.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA