Naslov
Assessment of the influence of different sample processing and cold storage duration on plant free proline content analyses

Autori
Teklić, Tihana ; Špoljarević, Marija ; Stanisavljević, Aleksandar ; Lisjak, Miroslav ; Vinković, Tomislav ; Parađiković, Nada ; Andrić, Luka ; Hancock, John

Izvornik
Phytochemical analysis (0958-0344) 21 (2010), 6; 561-565

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cold storage ; Fragaria x ananassa Duch. ; Glycine max (L.) Merr. ; hypocotyl ; liquid nitrogen ; leaf ; proline analyses ; sample processing

Sažetak
A method which is widely accepted for the analysis of free proline content in plant tissues is based on the use of 3% sulfosalicylic acid as an extractant, followed by spectrophotometric quantification of a proline ninhydrin complex in toluene. However, sample preparation and storage may influence the proline actually measured. This may give misleading or difficult to compare data. To evaluate free proline levels fresh and frozen strawberry (Fragaria x ananassa Duch.) leaves and soybean (Glycine max (L.) Merr.) hypocotyl tissues were used. These were ground with or without liquid nitrogen and proline extracted with sulfosalicylic acid. A particular focus was the influence of plant sample cold storage duration (1, 4 and 12 weeks at -20oC) on tissue proline levels measured. Results – The free proline content analyses, carried out in leaves of Fragaria x ananassa Duch. as well as in hypocotyls of Glycine max (L.) Merr., showed a significant influence of the sample preparation method and cold storage period. Long term storage of up to 12 weeks at -20oC led to a significant increase in the measured proline in all samples analyzed. The observed changes in proline content in plant tissue samples stored at -20oC indicate the likelihood of the over- estimation of the proline content if the proline analyses are delayed. Plant sample processing and cold storage duration seem to have an important influence on results of proline analyses. Therefore it is recommended that samples should be ground fresh and analyzed immediately.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

POVEZANOST RADA