Naslov
The role of IgG avidity in diagnosis of cytomegalovirus infection in newborns and infants

Autori
Vilibić Čavlek, Tatjana ; Ljubin-Sternak, Sunčanica ; Vojnović, Gordana ; Sviben, Mario ; Mlinarić-Galinović, Gordana

Izvornik
Acta microbiologica et immunologica Hungarica (1217-8950) 56 (2009), Suppl.; 259-260

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, znanstveni

Ključne riječi
cytomegalovirus ; IgG avidity ; infection in newborns and infants

Sažetak
Cytomegalovirus (CMV) is the most common cause of congenital viral infection. CMV IgM antibody detection is a good indicator of acute or recent primary infection. However, some congenitally infected babies do not produce IgM antibodies or IgM antibody production can be delayed. Determination of IgG avidity could help in determination of primary CMV infection. The aim of this study was to evaluate the value of IgG avidity in diagnosis of CMV infection in newborns and infants. Serum samples from 40 infants under 12 months of age with congenital/perinatal CMV infection were tested for IgM, IgG and IgG avidity using an enzyme-linked immunosorbent assay (ELISA). The determination of IgG avidity was carried out with urea as denaturing agent. For 25 of them, virus isolation and/or polymerase chain reaction (PCR) on urine specimens were performed. Thirteen (32.5%) patients showed the presence of CMV IgM antibodies, 3 (7.5%) had equivocal IgM result, and 24 (60.0%) patients had IgG antibodies only. Using IgG avidity, primary CMV infection (low avidity antibodies) was documented in 61.5% IgM positive and 54.2% IgM negative patients. Eight of nine (88.8%) IgM positive patients were positive either on virus isolation or PCR. In IgM negative patients, 46.6% urine cultures were positive for CMV and 66.6% were PCR positive. IgG avidity demonstrated acute/recent primary CMV infection in 58.8% patients younger than three months compared with 91.7% and 81.8% in 3-6 and 6-12 months old babies, respectively. In conclusion, IgG avidity can identify primary CMV infection either in IgM positive or IgM negative children older than 3 months of age. In infants less than 3 months, transplacentally derived maternal IgG antibodies of high avidity influence on the IgG avidity result. In these children, CMV infection should be confirmed by direct virologic methods such as virus isolation or PCR.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

Napomena
Collegium AntropologicumOpen AccessVolume 36, Issue 1, March 2012, Pages 297-300 https://hrcak.srce.hr/index.php?id_clanak_jezik=117099&show=clanak

POVEZANOST RADA