Naslov
Clinical and pathological findings of an outbreak of Tyzzer’s disease in a rabbit colony in Croatia

Autori
Artuković, Branka ; Grabarević, Željko ; Gudan Kurilj, Andrea ; Beck, Ana ; Shirota, Kinji ; Sakurada, Yosuke ; Kawamura, Shoji ; Džaja, Petar

Izvornik
Veterinarski arhiv (0372-5480) 80 (2010), 6; 761-770

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Tyzzer’s disease; Clostridium piliforme; rabbit; PCR; immunohistochemistry

Sažetak
Clinical and pathological findings found during the outbreak of Tyzzer’s disease in one rabbit colony in Croatia are described in this paper. The disease occurred in young rabbits, approximately 50 days old. During period of six months, 148 weaned rabbits from a total group of 1753 weaned animals died with symptoms of severe gastrointestinal disease. The main clinical signs observed were diarrhea, abdominal distension, anorexy, weight loss and apathy. Five weaned rabbits were examined at the Department of Pathology of Veterinary faculty. In all necropsed animals the severe entero-hepatic lesions characterized with disseminated hepatic focal necroses and transmural necrotic typhlitis were seen. Only one animal has necrotic myocarditis. For histopathology the tissue blocks were sectioned in 5m thick sections and stained using HE, PAS, GMS and Giemsa method. Immunohistochemistry using anti-Clostridium piliforme RT and MSK strain reacted positively within hepatocytes and intestine. Polymerase chain reaction (PCR) assay (liver and caecum) amplified the 196-bp DNA fragment specific to 16S ribosomal RNA of C. piliforme. It is the first confirmed and described case of Tyzzer’s disease in rabbit in Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA