Naslov
Does zinc influence SOD-like activity in different cell types in vitro?

Autori
Gavella, Mirjana ; Lipovac, Vaskresenija ; Vučić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International Society of Antioxidants in Nutrition & Health / - Pariz, 2000, 94-94

Skup
The 2 International Conference on Superoxide Dismutases

Mjesto i datum
Pariz, Francuska, 18.05.2000. - 19.05.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
zinc; SOD-like activity; cells

Sažetak
Activities of Cu, Zn superoxide dismutase (SOD) isolated from spermatozoa, polymorphonuclear leukocytes, erythrocytes and/or in intact cells exposed to different zinc (Zn) concetration were investigated. Cells were incubated (30 min, 37 C) in the presence of 20-1000 umol/l Zn and subsequently washed, lysed by the cold shock procedure and analysed for SOD-like activity. In other set of experiments, the enzyme was first extracted from cells by the cold shock and isolated SOD was then incubated in the presence of zinc under the same conditions. SOD-like activity was measured using xanthine/xanthine oxidase method. Cellular zinc contentwas measured using an atomic absorption spectrophotometer. Incubation with zinc resulted in a dose dependent decrease in spermatozoa SOD activity. The most pronounced decrease of SOD-like activity was obtained for enzyme permeabilized from spermatozoa and then exposed to 1000 umol/l Zn. The remaining activity amounted 47% of the activity without zinc (100%), while the remaining SOD-like activity of intact spermatozoa exposed to zinc was 63%. The SOD extracted from PMNs was significantly decreased at 20 umol/l zinc, while there was no change at 100 and 200 umol/l Zn. SOD of intact PMNs as well as SOD of intact and lysed human erythrocytes or commercial SOD from bovine erythrocytes (Sigma)remained unaffected by zinc. In conclusion, these results show that compared to other cell types studied, spermatozoa SOD was the most sensitive to in vitro inhibitory effect of zinc. On the other hand, SOD from erythrocytes was resistant to such influence, while the SOD from leukocytes was affected by zinc, only when extracted from the cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA