Naslov
Molecular characterization of citrus exocortis viroid RNA, a new pathogen of Croatian citrus cultivars

Autori
Škorić, Dijana ; Szychowski, Judith A. ; Semancik, Joseph S.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of the Silver Jubilee Meeting of the Croatian Biochemical Society (HB2000) / Floegel, Mirna - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2000

Skup
Silver Jubilee Meeting of the Croatian Biochemical Society (HB2000)

Mjesto i datum
Zagreb, Hrvatska, 13.10.2000. - 15.10.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
citrus exocortis viroid; sequence; structure; Croatia

Sažetak
Citrus exocortis viroid (CEVd) is a single-stranded circular RNA well known for its pathogenic effects on many cultivated plant species. Samples of various citrus cultivars (lemon, mandarin, orange) showing symptoms of viroid diseases (bark scalling, leaf yellowing and curling, dieback) were collected in the summer of 1998 from representative citrus-growing regions in Croatia. A biological test for the presence of viroids was made by transmission to the viroid sensitive citron selection Citrus medica L. 861-S1 with bud graft inoculation. In 5 out of 10 samples, CEVd was detected. All 5 CEVd-isolates showed severe symptoms on the experimental citron. Nucleic acids from apex tissues of citron, enriched for viroid RNA by LiCl partitioning and chromatography, were prepared and analyzed by sequencial polyacrylamide gel electrophoresis (sPAGE). The sPAGE analysis confirmed the presence of the viroid RNA in the samples. Viroid-enriched preparations were amplified by RT-PCR employing two pairs of CEVd-specific primers and the viroid cDNA was sequenced by the dideoxy termination technique. Croatian CEVd-variants were 371 nucleotide long and had a unique insertion in the upper strand of V-domain (nucleotide 129). A set of suboptimal secondary structures was determined by computer modelling. This is the first molecular evidence on the presence of CEVd in citrus cultivars grown in Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA