Naslov
Analysis of the Role of Protein Kinase C-alpha in HL-60 Cell Proliferation and Differentiation

Autori
Višnjić, Dora ; Batinić, Drago ; Banfić, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1. Kongres Hrvatskog društva fiziologa / Rukavina, Daniel - Osijek : Hrvatsko društvo fiziologa, 2000, P-6

Skup
1. Kongres Hrvatskog društva fiziologa

Mjesto i datum
Hrvatska, 14.09.2000. - 16.09.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
protein kinase-C; HL-60 cell; proliferation; differentiation
(protein kinase-C; HL-60 cell; proliferation; differantiation)

Sažetak
The role of protein kinase C (PKC) alpha in HL-60 cell proliferation and 1,25-dihidroxyvitamin D3 [1,25(OH)_2 D_3]-induced monocytic differentiation was investigated. The pretreatment of HL-60 cells with different PKC inhibitors abolished the 1,25(OH)_2 D_3-mediated effects on sphingomyelin hydrolysis, ceramide release and the activity of cytosolic, Mg^2^+-independent, neutral sphingomyelinase. Immunodepletion studies shown that of all classical, novel and atypical isoforms tested, only the depletion of PKCalphafrom cytosol completely inhibited the activation of sphingomyelinase induced by 1,25(OH)_2D_3. The most specific PKC inhibitors were further used in order to investigate the role of PKC in 1,25(OH)_2D_3-induced growth arrest and monocytic marker expression in HL-60 cells. The treatment of HL-60 cells with calphostin C, an inhibitor of classical and novel PKC isoforms, induced apoptosis. The pretreatment of HL-60 cells with Gö 6976, a specific inhibitor of PKCalpha and beta1, had no effects on 1,25(OH)_2D_3-mediated induction of CD14 expression. The treatment for 24 to 72 hours with Gö 6976 alone caused the progressive increase in the relative number of G1 DNA cells.

Izvorni jezik
Engleski

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