Naslov
Effect of creatine on erythrocyte rheology in vitro

Autori
Lipovac, Vaskresenija ; Gavella, Mirjana ; Vučić, Marijana ; Mrzljak, Vladimir ; Ročić, Boris

Izvornik
Clinical Hemorheology and Microcirculation (1386-0291) 22 (2000), 1; 45-52

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
creatine; erythrocyte filterability; red blood cell-lipid peroxidation; diabetes mellitus

Sažetak
To evaluate how creatine influences erythrocyte deformability, we determined its effect on erythrocyte filterability in 9 subjects with insulin-dependent diabetes (IDDM) without complications. 14 diabetics with uremia and 10 non-diabetic controls. The short-term incubation (15 min at 37 0C) of diabetic erythrocytes with 3 mM creatine improved cell filterability (assessed according to the reid method)from IDDM subjects without complications by 28.4% and that from diabetics with uremia by 18.9%. No rheological effect of creatine was found in erythrocytes from non-diabetic controls.However, a significant protective effect against erythrocyte filterability impairment induced by treatment of red blood cells from non-diabetic controls with hydrogen peroxide was observed with 3 mM (p<0.04) and 5 mM (p<0.01) creatine, respectively. Measurement of the thiobarbituric acid (TBA) reactivity was used to assess hydrogen peroxide induced formation of malondialdehyde (MDA). We found that creatine inhibits hydrogen peroxide-induced erythrocyte MDA-formation in a dose dependent manner by 20.4% 22.3% and 41.4% for 1, 3 aND 5 mM creatine, respectively. These results suggest that creatine by its ability to inhibit erytherocyte lipid peroxidation may contribute to the maintenence of normal cell deformability.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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