Naslov
Synthesis and in vitro behavior of gelatin/hydroxyapatite biocomposite

Autori
Kranželić, Dajana ; Ignjatić Zokić, Tatjana ; Ivanković, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
VIII. susret mladih kemijskih inženjera, knjiga sažetaka / Bolf, Nenad ; Šoljić Jerbić, Ivana - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2010

ISBN
978-953-6470-48-8

Skup
VIII. susret mladih kemijskih inženjera 2010.

Mjesto i datum
Zagreb, Hrvatska, 18.02.2010. - 19.02.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
hydroxyapatite scaffold; gelatin; composite; HBSS

Sažetak
Calcified tissue, such as bone and teeth, is basically considered as inorganic/organic hybrid consisting of ~70 wt.% hydroxyapatite (HAp, Ca10(PO4)6(OH)2) and 30 wt.% organic (largely collagen) constituents. In this work a two-stage route to prepare porous composite scaffold, suitable for bone graft application, has been involved. In the first stage, complete conversion of aragonite originated cuttlefish bone (Sepia officinalis L.) to pure HAp, maintaining the original porous micro-architecture of bone, was achieved. In the second stage, different concentration of gelatin (GEL) solution (5, 10, 20% w/v), derived from the organic constituent of bone (collagen), was impregnated into the HAp scaffolds and cross-linked with glutaraldehyde. HAp and HAp/GEL scaffolds were immersed in Hank`s balanced salt solution (HBSS) thermostated at 37°C for up to four weeks to evaluate their dissolution behavior. Hydrothermal conversion of aragonite (CaCO3) from cuttlefish bone into HAp was confirmed by XRD analysis. The FTIR spectra of HAp/GEL composites contain only the characteristic peaks of HAp and GEL. There is no evidence of formation new chemical bonds between HAp and GEL. The TGA curves showed two main weight losses centered approximately at 350°C and at 520 °C that are attributed to the elimination of proline and to the oxidation of all content of glyicine, respectively. Morphological investigations of the prepared composite scaffold performed by SEM revealed the appearance of much more regular sponge-like microspheres (5-10μm) deposited on the original microstructure of HAp converted cuttlefish bone. Gravimetric analysis of samples immersed in HBSS showed mineralization behavior of HAp and HAp/GEL composites. After initial mass increasement, HAp scaffold was partially crumbled with weight loss ~6.7 wt% during 28 days while HAp/GEL composite samples showed mass increasement ~7.0 wt% due to the Ca-P precipitation on composite surface which is confirmed by the results obtained from chemical analysis of HBSS solution.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne tehničke znanosti

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