Naslov
Synthesis and characterization of porous gelatin/hydroxyapatite composite

Autori
Kranželić, Dajana ; Jakovljević, Suzana ; Ivanković, Hrvoje ; Tkalčec, Emilija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International conference MATRIB 2009 Abstracts book / Grilec, Krešimir ; Marić, Gojko - Zagreb : Hrvatsko društvo za materijale i tribologiju (HDMT), 2009

Skup
MATRIB 2009

Mjesto i datum
Vela Luka, Hrvatska, 24.06.2009. - 26.06.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
hydroxyapatite scaffold; gelatin; composite; bone tissue engineering

Sažetak
The composition of human bone is well-known inorganic/organic hybrid consisting of ~70 wt.% hydroxyapatite (HAp, Ca10(PO4)6(OH)2) and 30 wt.% organic (largely collagen) constituents. In this work a two-stage route to prepare porous composite scaffold, suitable for bone graft application, has been involved. In the first stage, complete conversion of aragonite originated cuttlefish bone (Sepia officinalis L.) to pure HAp, maintaining the original porous micro-architecture of bone, was achieved. In the second stage, 5 wt. % gelatin solution (GEL), derived from the organic constituent of bone (collagen), was impregnated into the HAp scaffolds and cross-linked with glutaraldehyde. Morphology of the prepared porous HAp/GEL composite scaffold has been examined by SEM. The phase composition of the composites has been investigated by XRD, FTIR and TG analysis. The FTIR spectra of HAp/GEL composites contain only the characteristic peaks of HAp and GEL. There is no evidence of formation new chemical bonds between HAp and GEL. Different content of organic phase obtained by TG analysis, after heat treatment of the samples up to 1000oC, corresponds to the different procedure of impregnation. The TGA curves showed two main weight losses centered approximately at 350°C and at 520 °C that are attributed to the elimination of proline and to the oxidation of all content of glyicine, respectively. Morphological investigations of the prepared composite scaffold performed by SEM revealed the appearance of much more regular sponge-like microspheres deposited on the original microstructure of HAp converted cuttlefish bone. The EDX spectra of sponge-like microspheres indicated the presence of C(~18.8 wt.%), P(~14.8 wt.%)and Ca(~20.2 wt.%).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne tehničke znanosti

POVEZANOST RADA