Naslov
Antioxidative effect of melatonin on human spermatozoa

Autori
Gavella, Mirjana ; Lipovac, Vaskresenija

Izvornik
Archives of andrology (0148-5016) 44 (2000), 1; 23-27

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
human sperm; lipid peroxidation; melatonin; Trolox

Sažetak
The ability of melatonin to suppress experimentally induced lipid peroxidation (LPO) in sperm membrane was investigated in 41 samples of infertile men. Iron/ascorbate (0.04/0.2 mmol)-induced LPO was measured by the formation of malondialdehyde (MDA) using the thiobarbituric acid method. Sperm incubated in the presence of melatonin (2-6 mmol) exhibited a concnwetration-dependent decrease of MDA generated from hydroperoxide of the sperm plasma membrane in the presence of promoter system. Addition of 6 mmol of melatonin significantly reduced the rate of lipid peroxidation in sperm of unselected donors.Inhibory effect of melastonin was also significant in the presence of 0.015 mmol of ferrous ions. Comparing the effect of melatonin with that of Trolox , an analog of vitamin E, , a similasr effect at concentration of 0.1-0.2 mmol of Trolox was found.The obtained data of in vitro experiments show that melatonin is 40-fold less efficient that Trolox in achieving the 50% reduction in LPO (4 vs. 0.1 mmol).Since the physiological concnetration of melatonin in human semen is at the nanomolar level, its antioxidative role in vivo is probably of minor importance.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA