Naslov
Methods for Culture-Independent Identification of Lactic Acid Bacteria in Dairy Products

Autori
Pogačić, Tomislav ; Kelava, Nikolina ; Zamberlin, Šimun ; Dolenčić-Špehar, Iva ; Samaržija, Dubravka

Izvornik
Food technology and biotechnology (1330-9862) 48 (2010), 1; 3-10

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
culture-independent identification; molecular methods; lactic acid bacteria; dairy products

Sažetak
Culture-independent molecular tools have been introduced into food microbiology during the last ten years. Most of them are based on the amplification of a bulk bacterial DNA extracted directly from a sample, the targeting of a selected gene, or a variable region of the selected gene. Many studies have explored indigenous lactic acid bacteria in dairy products by culture-independent molecular approaches. It is well known that indigenous microbiota significantly contribute to the uniqueness of artisanal cheeses. However, there is no molecular method that can provide complete qualitative and quantitative insight into the microbiota associated with a certain ecosystem. Therefore, a combination of molecular approaches should be applied to get a more objective picture of the microbiota. This paper aims to present the most widely used culture-independent molecular tools for identifying lactic acid bacteria in dairy products.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA