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Pregled bibliografske jedinice broj: 444763

Comparisons of mumps virus potency estimates obtained by 50% cell culture infective dose assay and plaque assay

Forčić, Dubravko; Košutić-Gulija, Tanja; Šantak, Maja; Jug, Renata; Ivančić-Jelečki, Jelena; Markušić, Maja; Mažuran, Renata
Comparisons of mumps virus potency estimates obtained by 50% cell culture infective dose assay and plaque assay // Vaccine, 28 (2010), 7; 1887-1892 doi:10.1016/j.vaccine.2009.11.049 (međunarodna recenzija, članak, znanstveni)

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Naslov
Comparisons of mumps virus potency estimates obtained by 50% cell culture infective dose assay and plaque assay

Autori
Forčić, Dubravko ; Košutić-Gulija, Tanja ; Šantak, Maja ; Jug, Renata ; Ivančić-Jelečki, Jelena ; Markušić, Maja ; Mažuran, Renata

Izvornik
Vaccine (0264-410X) 28 (2010), 7; 1887-1892

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Mumps virus; Cytophatic effect; Virus potency estimate

Sažetak
The two most commonly used methods for the determination of a virus potency are plaque assay and 50% cell culture infective dose (CCID50) assay, both based on cytopathic effect observation. We compared the potency estimates obtained by plaque and CCID50 assays for nine mumps virus strains that produce different cytopathic effects in Vero cells. The ratios of CCID50 and plaque assay quantification results differed for different strains and were in a range of 0.66–10, indicating that quantification results for some mumps virus strains are almost identical regardless of whether CCID50 or plaque method is used, while the potency estimates of other strains strongly depend on the choice of the assay.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija



POVEZANOST RADA

Projekti:
TP-05/0021-02
021-0212432-3123 - Molekularna patogeneza virusa mumpsa (Šantak, Maja, MZOS ) ( CroRIS)

Ustanove:
Imunološki zavod d.d.

Profili:

Avatar Url Maja Šantak (autor)

Avatar Url Jelena Ivančić-Jelečki (autor)

Avatar Url Maja Jagušić (autor)

Avatar Url Renata Mažuran (autor)

Avatar Url Dubravko Forčić (autor)

Avatar Url Tanja Košutić Gulija (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com www.sciencedirect.com

Citiraj ovu publikaciju:

Forčić, Dubravko; Košutić-Gulija, Tanja; Šantak, Maja; Jug, Renata; Ivančić-Jelečki, Jelena; Markušić, Maja; Mažuran, Renata
Comparisons of mumps virus potency estimates obtained by 50% cell culture infective dose assay and plaque assay // Vaccine, 28 (2010), 7; 1887-1892 doi:10.1016/j.vaccine.2009.11.049 (međunarodna recenzija, članak, znanstveni)
Forčić, D., Košutić-Gulija, T., Šantak, M., Jug, R., Ivančić-Jelečki, J., Markušić, M. & Mažuran, R. (2010) Comparisons of mumps virus potency estimates obtained by 50% cell culture infective dose assay and plaque assay. Vaccine, 28 (7), 1887-1892 doi:10.1016/j.vaccine.2009.11.049.
@article{article, author = {For\v{c}i\'{c}, Dubravko and Ko\v{s}uti\'{c}-Gulija, Tanja and \v{S}antak, Maja and Jug, Renata and Ivan\v{c}i\'{c}-Jele\v{c}ki, Jelena and Marku\v{s}i\'{c}, Maja and Ma\v{z}uran, Renata}, year = {2010}, pages = {1887-1892}, DOI = {10.1016/j.vaccine.2009.11.049}, keywords = {Mumps virus, Cytophatic effect, Virus potency estimate}, journal = {Vaccine}, doi = {10.1016/j.vaccine.2009.11.049}, volume = {28}, number = {7}, issn = {0264-410X}, title = {Comparisons of mumps virus potency estimates obtained by 50\% cell culture infective dose assay and plaque assay}, keyword = {Mumps virus, Cytophatic effect, Virus potency estimate} }
@article{article, author = {For\v{c}i\'{c}, Dubravko and Ko\v{s}uti\'{c}-Gulija, Tanja and \v{S}antak, Maja and Jug, Renata and Ivan\v{c}i\'{c}-Jele\v{c}ki, Jelena and Marku\v{s}i\'{c}, Maja and Ma\v{z}uran, Renata}, year = {2010}, pages = {1887-1892}, DOI = {10.1016/j.vaccine.2009.11.049}, keywords = {Mumps virus, Cytophatic effect, Virus potency estimate}, journal = {Vaccine}, doi = {10.1016/j.vaccine.2009.11.049}, volume = {28}, number = {7}, issn = {0264-410X}, title = {Comparisons of mumps virus potency estimates obtained by 50\% cell culture infective dose assay and plaque assay}, keyword = {Mumps virus, Cytophatic effect, Virus potency estimate} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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