Pregled bibliografske jedinice broj: 444428
Epidermal growth factor receptor protein expression and gene amplification in normal, hyperplastic, and cancerous glottic tissue: immunohistochemical and fluorescent in situ hybridization study on tissue microarrays.
Epidermal growth factor receptor protein expression and gene amplification in normal, hyperplastic, and cancerous glottic tissue: immunohistochemical and fluorescent in situ hybridization study on tissue microarrays. // Croatian medical journal, 50 (2009), 4; 370-379 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 444428 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Epidermal growth factor receptor protein expression and gene amplification in normal, hyperplastic, and cancerous glottic tissue: immunohistochemical and fluorescent in situ hybridization study on tissue microarrays.
Autori
Braut, Tamara ; Krstulja, Mira ; Kujundžić, Milodar ; Manestar, Dubravko ; Hadžisejdić, Ita ; Jonjić, Nives ; Grahovac, Blaženka ; Manestar, Darko
Izvornik
Croatian medical journal (0353-9504) 50
(2009), 4;
370-379
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
EGFR; quantitative immunohistochemistry; FISH; glottic cancer; tissue microarray; EGFR targeted therapy
Sažetak
To evaluate the importance of epidermal growth factor receptor (EGFR) protein overexpression and gene amplification in carcinogenesis of glottic cancer. In order to evaluate EGFR expression at protein and gene level, immunohistochemical (IHC) analysis and fluorescent in situ hybridization (FISH) were performed on tissue microarrays of laryngeal tissue (145 samples) -- 38 samples of normal mucosa, 46 samples of hyperplastic lesions, and 61 samples of cancerous lesions. Membranous (mEGFR) and cytoplasmic (cEGFR) EGFR expression was significantly different between the analyzed groups. The differences were most striking in the suprabasal-transforming zone. IHC evaluation showed that high and low mEGFR staining contributed to the differentiation of dysplastic lesions, simple hyperplasia, and cancerous tissue, as well as between different degrees of atypia in hyperplastic lesions (P<0.050). EGFR gene amplification was not found in simple and abnormal hyperplastic lesions, but it was confirmed in 2/21 atypical hyperplasias, indicating that gene amplification can facilitate identification of malignant potential in hyperplastic lesions. In cancerous tissue, EGFR gene amplification was found in 8/50 samples. EGFR gene amplification was found in preinvasive cancer in one patient. In invasive carcinomas, gene amplification was not associated with stage or grade. Carcinomas with gene amplification showed significantly higher cEGFR expression (basal layer P=0.003 ; suprabasal layer P=0.002). This study confirmed an increase in EGFR protein expression and gene amplification with the increase in biological aggressiveness of glottic lesions. A correlation between EGFR gene amplification and protein expression was established. Gene amplification proved to be an early event in glottic carcinogenesis, indicating its importance for glottic cancer prevention, early detection, and protocol selection.
Izvorni jezik
Engleski
POVEZANOST RADA
Projekti:
062-0620095-0083 - Uloga EGFR-a u patogenezi karcinoma larinksa (Manestar, Darko, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Blaženka Grahovac
(autor)
Darko Manestar
(autor)
Milodar Kujundžić
(autor)
Mira Krstulja
(autor)
Nives Jonjić
(autor)
Ita Hadžisejdić
(autor)
Tamara Braut
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE