Naslov
Cyp 4a14 overexpression induced by hyperoxia in female CBA mice as a possible contributor of increased resistance to oxidative stress

Autori
Sobočanec, Sandra ; Balog, Tihomir ; Šarić, Ana ; Šverko, Višnja ; Žarković, Neven ; Čipak Gašparović, Ana ; Žarković, Kamelija ; Waeg, Georg ; Mačak Šafranko, Željka ; Kušić, Borka ; Marotti, Tatjana

Izvornik
Free radical research (1071-5762) 44 (2010), 2; 181-190

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Antioxidative enzymes Cyp 4a14; HNE; Lipid peroxidation; Mice

Sažetak
The beneficial effects of hyperoxia have been noted in treatment of several diseases and pathological states. However, the excessive production of ROS under hyperoxic conditions can directly damage cellular macromolecules if the imbalance in antioxidant status exists. Cytochrome P450 (Cyp) 4a14 has an important role in the metabolism of lipids and as a source of ROS in oxidative stress. This study investigated the oxidant/antioxidant status as a response to hyperoxia treatment in liver of young CBA/Hr mice of both sexes and whether the observed response is mediated by Cyp4a14 via PPAR isoforms in a sex-dependent manner. The overexpression of Cyp4a14, lack of both LPO and of 4-hydroxynonenal(HNE)-protein adducts revealed by immunohistochemical analysis in hyperoxia-treated females indicates their greater resistance to hyperoxia compared to males, which is parallelled to changes in PPARβ/δ and PPARγ expression. These results suggest the presence of sex-dependent changes in all investigated parameters, which points out sex-related susceptibility towards oxidative stress and hyperoxia treatment of various pathological conditions and diseases.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
Rad je kao poster prezentiran na skupu Lipid Oxidation, Human Diseases And Ageing, održanom od 15.-19.06.2010., Torino, Italija ; objavljen u Knjizi sažetaka.

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