Naslov
Influence of microencapsulation and freeze-drying on the functionality of probiotic bacteria

Autori
Beganović, Jasna ; Kos, Blaženka ; Leboš Pavunc, Andreja ; Dukić, Mario ; Radoš, Ivan ; Šušković, Jagoda

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
2nd Central European Forum for Biotechnology

Mjesto i datum
Keszthely, Mađarska, 07.10.2009. - 09.10.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
funkcionalnost; liofilizacija; lioprotektori; mikroinkapsulacija; probiotic
(functionallity; lyophilisation; lyoprotectors; microencapsulation; probiotic)

Sažetak
Bacterial strains Lactobacillus helveticus M92, Lactobacillus plantarum L4 and Enterococcus faecium L3 have been previously characterised as probiotic strains according to strong probiotic selection criteria in Laboratory for Antibiotic, Enzyme, Probiotic and Starter Culture Technology on Faculty of Food Technology and Biotechnology University of Zagreb. Besides their traditional applications as functional starter cultures for various food commodities, there is also a great interest for an investigation of these bacteria as biotherapeutic agents. The major criterion for the selection of probiotics is their survival during the transit through gastrointestinal tract. In order to exert their health benefits, probiotics need to be delivered to a host at high viable cell number, as a concentrated powder, that could be performed by freeze-drying. Hence, the objective of this work was to investigate the effects of different lyoprotectants on the survival of probiotic strains L. helveticus M92, L. plantarum L4 and E. faecium L3 during freeze-drying. Fresh and freeze-dried bacterial cells were microencapsulated in alginate and the effect of the microencapsulation on a viability of these probiotic bacteria in the simulated gastrointestinal tract conditions was investigated. The highest viable bacterial cells counts were reached when skim milk with glycerol were applied as lyoprotectants. The better survival of microencapsulated than non-microencapsulated probiotic cells, in simulated gastrointestinal tract conditions, indicates the protective effect of the microencapsulation in alginate. The presence of pure probiotic culture in each examined freeze-dried preparation was confirmed by RAPD (Randomly Amplyfied Polymorphic DNA).

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA