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Pregled bibliografske jedinice broj: 442482

Deletion of IglH in virulent Francisella tularensis subsp. holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain

Straskova, Adela; Cerveny, Lukas; Spidlova, Petra; Dankova, Vera; Belčić, Davor; Šantić, Marina; Stulik, Jiri
Deletion of IglH in virulent Francisella tularensis subsp. holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain // Microbes and infection, 14 (2012), 2; 177-187 doi:10.1016/j.micinf.2011.08.017 (međunarodna recenzija, članak, znanstveni)

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Naslov
Deletion of IglH in virulent Francisella tularensis subsp. holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain

Autori
Straskova, Adela ; Cerveny, Lukas ; Spidlova, Petra ; Dankova, Vera ; Belčić, Davor ; Šantić, Marina ; Stulik, Jiri

Izvornik
Microbes and infection (1286-4579) 14 (2012), 2; 177-187

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Francisella; Macrophage; Phagosome; Mice; Protection

Sažetak
Francisella tularensis, the causative agent of tularemia, is a highly infectious intracellular pathogen with no licensed vaccine available today. The recent search for genome sequences involved in F. tularensis virulence mechanisms led to the identification of the 30-kb region defined as a Francisella pathogenicity island (FPI). In our previous iTRAQ study we described the concerted upregulation of some FPI proteins in different F. tularensis strains cultivated under stress conditions. Among them we identified the IglH protein whose role in Francisella virulence has not been characterized yet. In this work we deleted the iglH gene in a European clinical isolate of F. tularensis subsp. holarctica FSC200. We showed that the iglH gene is necessary for intracellular growth and escape of F. tularensis from phagosomes. We also showed that the iglH mutant is avirulent in a mouse model of infection and persists in the organs for about three weeks after infection. Importantly, mice vaccinated by infection with the iglH mutant were protected against subcutaneous challenge with the fully virulent parental FSC200 strain. This is the first report of a defined subsp. holarctica FPI deletion strain that provides protective immunity against subsequent subcutaneous challenge with a virulent isolate of F. tularensis subsp. holarctica.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA

Projekti:
062-0621273-0950 - Francisella tularensis-unutarstanični život i patogeneza tularemije u miša (Šantić, Marina, MZOS ) ( CroRIS)
062-0621273-1275 - Patogeneza eksperimentalne legioneloze (Dorić, Miljenko, MZOS ) ( CroRIS)

Ustanove:
Medicinski fakultet, Rijeka

Profili:

Avatar Url Marina Šantić (autor)

Poveznice na cjeloviti tekst rada:

doi ac.els-cdn.com www.sciencedirect.com dx.doi.org

Citiraj ovu publikaciju:

Straskova, Adela; Cerveny, Lukas; Spidlova, Petra; Dankova, Vera; Belčić, Davor; Šantić, Marina; Stulik, Jiri
Deletion of IglH in virulent Francisella tularensis subsp. holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain // Microbes and infection, 14 (2012), 2; 177-187 doi:10.1016/j.micinf.2011.08.017 (međunarodna recenzija, članak, znanstveni)
Straskova, A., Cerveny, L., Spidlova, P., Dankova, V., Belčić, D., Šantić, M. & Stulik, J. (2012) Deletion of IglH in virulent Francisella tularensis subsp. holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain. Microbes and infection, 14 (2), 177-187 doi:10.1016/j.micinf.2011.08.017.
@article{article, author = {Straskova, Adela and Cerveny, Lukas and Spidlova, Petra and Dankova, Vera and Bel\v{c}i\'{c}, Davor and \v{S}anti\'{c}, Marina and Stulik, Jiri}, year = {2012}, pages = {177-187}, DOI = {10.1016/j.micinf.2011.08.017}, keywords = {Francisella, Macrophage, Phagosome, Mice, Protection}, journal = {Microbes and infection}, doi = {10.1016/j.micinf.2011.08.017}, volume = {14}, number = {2}, issn = {1286-4579}, title = {Deletion of IglH in virulent Francisella tularensis subsp. holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain}, keyword = {Francisella, Macrophage, Phagosome, Mice, Protection} }
@article{article, author = {Straskova, Adela and Cerveny, Lukas and Spidlova, Petra and Dankova, Vera and Bel\v{c}i\'{c}, Davor and \v{S}anti\'{c}, Marina and Stulik, Jiri}, year = {2012}, pages = {177-187}, DOI = {10.1016/j.micinf.2011.08.017}, keywords = {Francisella, Macrophage, Phagosome, Mice, Protection}, journal = {Microbes and infection}, doi = {10.1016/j.micinf.2011.08.017}, volume = {14}, number = {2}, issn = {1286-4579}, title = {Deletion of IglH in virulent Francisella tularensis subsp. holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain}, keyword = {Francisella, Macrophage, Phagosome, Mice, Protection} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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