Naslov
Hepatic and Pancreatic Glycosphingolipid Phenotypes of the Neurological Different Rat Strains

Autori
Rešić, Jasminka ; Čikeš-Čulić, Vedrana ; Zemunik, Tatijana ; Markotić, Anita

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
THE THIRD CROATIAN CONGRESS OF NEUROSCIENCE / Kostović, Ivica - , 2009, 58-59

Skup
The third Croatian congress of neuroscience

Mjesto i datum
Zadar, Hrvatska, 24.09.2009. - 26.09.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
b-series gangliosides; GM3; GD1a; liver; pancreas; rat strains

Sažetak
Among three commonly used strains of laboratory rats, Wistar rats performe more neurological tasks better then Lewis and Sprague–Dawley (SD) rats. Liver is the main site of insulin-like growth factor (IGF) production and pancreas is the exclusive site of insulin production. Insulin stimulates neuronal development and appropriate IGF-I input is critical in brain growth. Glycosphingolipids (GSLs) are important mediators of insulin secretion and action. Therefore, this study investigated GSL phenotypes of liver and pancreas with hypothesis that they are different in three rat strains. Total GSL fractions (neutral and gangliosides) were analysed by high performance thin-layer chromatography (HPTLC). Complex gangliosides were detected by HPTLC immunostaining using cholera toxin B subunit after neuraminidase pretreatment.Wistar rats had the highest liver weight/body weight ratio and SD rats had the highest pancreas weight/body weight ratio. Ganglioside GM3 was more expressed in the liver of Wistar compared to Lewis and SD rats. SD rats contained scarce quantities of GD1a and b-series gangliosides in the liver. Pancreatic b-series ganglioside content was also low in SD rats. This study represents differences in the hepatic and pancreatic ganglioside phenotypes of three rat strains which have to be considered by the planning of neurochemical investigations linked to ganglioside expression.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA