Naslov
Spatial distribution of DNA integrity in mussels, Mytilus galloprovincialis, from the Adriatic Sea, Croatia

Autori
Bihari, Nevenka ; Fafanđel, Maja ; Jakšić, Željko ; Pleše, Bruna ; Batel, Renato

Izvornik
Bulletin of environmental contamination and toxicology (0007-4861) 75 (2005), 5; 845-850

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA ; damage ; mussel

Sažetak
The marine environment is the major ultimate recipient of increasing range of anthropogenic contaminants, a large proportion of which are genotoxic. The first event in marine organisms following exposure to genotoxic contaminants is the impact on the integrity of cellular DNA. Therefore, DNA integrity in certain marine organism reflects the level of marine pollution by genotoxins and is widely accepted as a good biomarker (Shugart 2000). Genotoxicity biomarkers detected in mussel Mytilus galloprovincialis have been validated in the field and applied on a large scale in coastal monitoring studies (Steinert et al. 1998, Rank 1999, Izquierdo 2003). DNA single strand brakes, as the most used genotoxic biomarker, have been detected by methods such as the DNA unwinding test (Nacci et al. 1992), alkaline elution (Vukmirović et al. 1994), and the Comet assay (Lee and Steinert 2003), revealing difference in sensitivity and discriminatory power. However, these methods do not satisfy the need for a fast, routine and simple technique for large numbers and series of samples. The Fast Micromethod® ; ; (Batel et al. 1999) detects DNA damage (strand breaks, alkali-labile sites and incomplete excision repair) and determines DNA integrity in cell suspension or tissue homogenates in single microplates. The sensitivity and precision of the method is similar to the Comet assay (Bihari et al. 2002). It was successfully applied for DNA integrity determination in dab (Lacorn et al. 2001) and marine invertebrates (Jakšić and Batel 2003) as well as in a long-term field study (Jakšić et al. 2005). Here we presented the results of genotoxic biomonitoring by the Fast Micromethod® ; ; performed in June 2001 using gills as the target organ from a sentinel organism the mussel Mytilus galloprovincialis, collected along the Adriatic coast, Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Geologija

POVEZANOST RADA