Naslov
Effect of occupational exposure to multiple pesticides on translocation yield and chromosomal aberrations in lymphocytes of plant workers

Autori
Želježić, Davor ; Lucić Vrdoljak, Ana ; Lucas, Joe N. ; Lasan, Ružica ; Fučić, Aleksandra ; Kopjar, Nevenka ; Katić, Jelena ; Mladinić, Marin ; Radić, Božica

Izvornik
Environmental Science and Technology (0013-936X) 43 (2009), 16; 6370-6377

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Plant workers; Occupational exposure; Chromosomal aberrations

Sažetak
Employees handling pesticides are simultaneously exposed to different active substances. Occurring multiple chemical exposures may pose a higher risk than it could be deduced from studies evaluating the effect of a single substance. This study comprised 32 pesticide plant workers exposed to carbofuran, chlorpyrifos, metalaxyl, and dodine and an equal number of control subjects. Groups were matched by age (43.8 ± 10.16 vs 41.8 ± 7.42, respectively), sex (14 females ; 18 males), and smoking (11 smokers ; 21 nonsmokers). Chromosome aberration and translocation frequencies were determined using a standard aberration assay and fluorescent in situ hybridization (FISH) by applying painting probes for chromosomes 1, 2, and 4. Although significant, an observed increase in chromatid breaks (5.2 ± 2.49) compared to controls (2.1 ± 0.87), pPostHoc = 0.000001 is biologically irrelevant. Genomic frequency of translocations was also significantly elevated (exposed 0.0165 ± 0.0070 ; control 0.0051 ± 0.0023, pPostHoc = 0.0000004). The distribution of translocations among chromosomes 1, 2, and 4 did not differ from control subjects. It corresponded to the distribution of DNA content among selected chromosomes indicating randomness of DNA damage. A good translocation yield correlation within years spent in pesticide production indicates that multiple pesticide exposure may pose a risk to genome integrity. However, for more accurate health risk assessments, the use of probes for some other groups of chromosomes should be considered.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti

POVEZANOST RADA