Naslov
Crystallography in bioanalysis

Autori
Matković-Čalogović, Dubravka ; Milić, Dalibor ; Đilović, Ivica ; Prugovečki, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts, CEEPUS International Symposium and Summer School on Bioanalysis / T. Pajpanova, T. Dzimbova - Blagoevgrad, 2009

Skup
CEEPUS International Symposium and Summer School on Bioanalysis

Mjesto i datum
Blagoevgrad, Bugarska, 06.09.2009. - 12.09.2009

Vrsta sudjelovanja
Plenarno

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
crystallography; bioanalysis

Sažetak
For characterization of a compound, either a small molecule or a macromolecule, information about structure and properties is needed. If the compound can be crystallized then X-ray diffraction analysis gives a lot of information about the molecular and crystal structure. Crstallization of proteins is difficult and can be a bottleneck for such analysis. Our newest results in protein crystallography will be briefly discussed: (1) enzymatic activity of tyrosine phenol-lyase (TPL) could be deduced by detailed structural analysis of native and mutated TPL forms in complexes with substrate analogues ; (2) 3-hydroxyanthranilate 3, 4-dioxygenase, enzyme that catalyzes the conversion of 3-hydroxyanthranilate to quinolinic acid, has been extracted and purified from bovine kidney, crystallized and its structure determined at 2.5 &Aring ; resolution ; (3) coordination of iron in bovine insulin changed upon addition of bromide or iodide ions into crystallization solutions.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA