Naslov
Myricetin, a naturally occuring flavonoid, prevents D-glucose induced oxidative damage in Hep G2 cells
(Myricetin, a naturally occuring flavonoid, prevents D-glucose induced oxidative damage in)

Autori
Petlevski, Roberta ; Frank, Saša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biochemia Medica / Topić, Elizabeta ; Čvorišćec, Dubravka - Zagreb : Hrvatsko društvo medicinskih biokemičara (HDMB), 2009, S131-S131

Skup
6th Croatian Congress of Medical Biochemists with international participation, Supetar, Brač, Croatia, September 30 - October 4, 2009

Mjesto i datum
Supetar, Hrvatska, 30.09.2009. - 04.10.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Miricetin; Diabetes mellitus; Glucose; oxidative damage

Sažetak
Hyperglicemia in diabetes can induce oxidative stress in Hep G2 cells via several mechanisms. These include glucose autoxidation, the formation of advanced glycation end-products (AGE), and activation of the polyol pathway. Myricetin is a flavonoid compound found in many fruits and vegetables, including red wine, that acts as a powerful antioxidant. The aim of the present study was to investigate effect of the flavonoid myricetin (Sigma, Co) within a low concentration range (5 x 10-7 and 1 x 10-6 M) on the concentration of total glutathione (GSH) in Hep G2 cells. The major roles of GSH ( -glutamiylcysteinylglycine) are to maintain the intracellular redox balance and to eliminate ROS in cells. Compared with control incubation, D-glucose (20 mM) significantly (p 0.05) lowering GSH content in Hep G2 cells(n=9). Exposure the Hep G2 cells plus glucose to low dose myricetin (5 x 10-7 M) for 4 hours at 37 degrees C resulted in significant increased of the GSH level (p 0.05) when compared with control cells. These results demonstrate that myricetin in low concentration attenuates D-glucose induced damage in Hep G2 cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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