Naslov
Hepatic metallothioneins expression and tissue metals kinetics in chronic relapsing form of experimental autoimmune encephalomyelitis in rats

Autori
Jakovac, Hrvoje ; Tota, Marin ; Grebić, Damir ; Marinić, Jelena ; Broznić, Dalibor ; Grubić Kezele, Tanja ; Barac-Latas, Vesna ; Milin, Čedomila ; Radošević-Stašić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Rabatić Sabina et al. - Zagreb : Hrvatsko imunološko društvo, 2009, 30-30

Skup
2009 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Starigrad, Hrvatska, 01.10.2009. - 04.10.2009

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
chronic relapsing EAE; metallothioneins I+II; tissue metals; liver

Sažetak
Experimental autoimmune encephalomyelitis (EAE) is an autoimmune mediated inflammatory demyelinating disease of the central nervous system whose pathology is similar to that of multiple sclerosis in humans. The disease is characterized by infiltration of myelin reactive Th1 or Th17 lymphocytes and macrophages, high-level expression of pro-inflammatory cytokines and glial activation and dysfunction, but the mechanisms involved in acute attacks and remissions are still not fully understood. It seems, however, that high anti-inflammatory and neuroprotective effects have also the low molecular cysteine-rich heavy metal binding proteins-metallothioneins (MTs), which act as antioxidants and as the immunosuppressive agents in instances of antigen-dependent adaptive immunity. Their local expression might be induced by reactive astrocytes, but since the neuroprotective molecular pathway might be activated also by extracellular and intraperitoneally applied MTs, the participation of MTs from other sources could not be excluded. Owing to the fact that the highest concentration of MTs might be found in the liver, which promptly reacts on oxidant damage, inflammatory mediators and metabolic dysbalances by acute phase reaction and high activation of hepatic immune system, in this study we analyzed the hepatic MTs expression, as well as the tissue kinetics of Zn2+, Cu2+ and Fe2+ during different clinical phases of chronic relapsing form of EAE, induced in the genetically susceptible DA rats. The data obtained by immunohistochemistry and inductivity coupled plasma spectrometry revealed that hepatic expression of MTs was markedly enhanced during the time of attacks (on the 12th and particularly on the 22nd post-immunization day). Moreover, during the both attacks of EAE in the liver significantly arose the concentrations Zn2+, Cu2+ and Fe2+ (p<0.0003 in comparison with CFA-treated rats), pointing to the direct participation of the liver in the pathogenesis of CR-EAE.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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